Publication:
The study of multiple extraction methods of phytochemical constituents of senna alata leaves

dc.contributor.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#en_US
dc.contributor.authorNadirah Abd Rahimen_US
dc.contributor.supervisorNur Sabrina Ahmad Azmi, Ph.Den_US
dc.contributor.supervisorMaizatul Akma Ibrahim, Ph.Den_US
dc.contributor.supervisorSahena Ferdosh, Ph.Den_US
dc.date.accessioned2024-10-09T07:48:04Z
dc.date.available2024-10-09T07:48:04Z
dc.date.issued2024
dc.description.abstractThe demand for “Gelenggang” scientifically named Senna alata Linn-based product, is increasing due to the well-known therapeutic properties that can treat various diseases such as skin disease, ringworm, and constipation. Various extraction methods have been used to study the active ingredients and find scientific evidence for their herbal activities. Thus, this study aims to investigate the difference in yield percentage, phytochemicals, antioxidant and antimicrobial activities of different extracts of S. alata leaf. The extraction methods used in this study were maceration, Soxhlet, and Subcritical Carbon Dioxide Soxhlet (SCDS), our findings suggested that conventional methods yield higher percentages of extracts compared to SCDS ranging from (1.20 ± 0.417e %) to (25.14 ± 1.017a %) with methanol extraction showed higher yields than hexane extraction, indicating the solvent's influence on extraction efficiency. The study also explores the effect of sample: co-solvent ratio on the yield of extracts using SCDS (1.20 ± 0.417e to 2.45 ± 0.417e %). GCMS analysis reveals the presence of eighteen compounds, with phytol acetate, dihydroactinidiolide, and hexadecenoic acid methyl ester identified as major constituents. The extracts exhibit in vitro antioxidant activity by the DPPH assay with the SCDS extract using methanol as the soaking solvent showing the strongest potency (IC50 value = 0.693 ± 0.1725 mg/ml), while the weakest also from SCDS method but ethanol as co-solvent with highest IC50 value (55.569 ± 2.29 mg/ml). On the other hand, agar disc diffusion assay resulted that methanolic extracts from Soxhlet showed the most significant inhibition zones against Staphylococcus aureus ATCC25923 which ranges from 9 to 11 mm. Furthermore, the resazurin-based microdilution techniques yielded the lowest MIC and MBC value from both methanolic Soxhlet extracts against K. pneumoniae (20.75 mg/ml). Overall, this research highlights the potential of SCDS as a promising extraction method for S. alata leaves, with potential to preserve bioactive constituents despite lower yield percentages compared to conventional methods.en_US
dc.description.abstractarabicFormat not supporteden_US
dc.description.cpsemailcps2u@iium.edu.myen_US
dc.description.degreelevelMaster
dc.description.emailnadirah960913@gmail.comen_US
dc.description.identifierThesis : The study of multiple extraction methods of phytochemical constituents of senna alata leaves / by Nadirah binti Abd Rahim [en_US
dc.description.identityG2025928Nadirahabdrahimen_US
dc.description.kulliyahKulliyyah of Scienceen_US
dc.description.nationalityMalaysianen_US
dc.description.notesThesis (MSC)--International Islamic University Malaysia, 2024.en_US
dc.description.physicaldescription1 online resource (xvi, 111 leaves) ; color illustrations.en_US
dc.description.programmeMaster of Scienceen_US
dc.identifier.urihttps://studentrepo.iium.edu.my/handle/123456789/11458
dc.language.isoenen_US
dc.publisherKuantan, Pahang : Kulliyyah of Science, International Islamic University Malaysia, 2024en_US
dc.rightsJOINTLY OWNED WITH A THIRD PARTY(S) AND/OR IIUM
dc.titleThe study of multiple extraction methods of phytochemical constituents of senna alata leavesen_US
dc.typeMaster Thesisen_US
dspace.entity.typePublication

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
G2025928Nadirahabdrahim_SEC.pdf
Size:
16.26 MB
Format:
Adobe Portable Document Format
Description:
Full text

Collections