Publication:
Antimicrobial activities of selected sponges and corals of costal waters of Malaysia

dc.contributor.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#en_US
dc.contributor.authorQaralleh, Haithamen_US
dc.date.accessioned2024-10-09T07:41:03Z
dc.date.available2024-10-09T07:41:03Z
dc.date.issued2012
dc.description.abstractAntibacterial pharmaceutical research groups are paying more attention to find novel antibiotics that could potentially overcome the development of bacterial resistance for the conventional antibiotics. In this study, four sponge species and three hard coral species were collected and identified based on their morphology, skeleton, and spicules characteristics. The sponge species investigated were Neopetrosia exigua, Xestospongia testudinaria (Langkawi), Xestospongia testudinaria (South China Sea) and Xestospongia sp. while the hard coral species investigated were Favites sp., Galaxea fascicularis, and Galaxea astreata. The collected species were extracted with deionized water followed by dichloromethane and methanol. The extracts were screened using disc diffusion and microdilution methods for antimicrobial activity against six pathogenic microorganisms. The result showed that aqueous and organic extracts of N. exigua exhibited potent activity against Bacillus cereus (inhibition zone 25 mm and MIC value of 0.07 μg/mL), Staphylococcus aureus (17.5 mm and 0.12 μg/mL) and against Candida albicans (21 mm and 0.32 μg/mL). N. exigua biomass extract was then subjected to bioassay guided isolation using sequential gradient partitioning with normal and reverse phase chromatography. From the polar fractions, four potent compounds were isolated, for the first time from N. exigua, hyrtiosine (34 μg/mL), demethylcystalgerone (11 μg/mL), xestospongien (11 μg/mL) and bisulfate avarol derivative (RnD1c) which was reported as a new natural product with potent bacteriostatic (2.6 μg/mL) and bactericidal (5.2 μg/mL) activities against S. aureus. The mode of action of RnD1c against S. aureus was investigated using a combination approach. Scanning Electron Microscope showed that RnD1c caused severe cell surface alteration of S. aureus. As a result of treating S. aureus with RnD1c, autolytic enzymes were triggered, N-acetylglucosamin synthesis was inhibited, membrane permeability was increased, leakage of ions was induced, intracellular ATP was decreased, arginine dihydrolase was inhibited and membrane depolarization was induced. The results imply that RnD1c exhibited multiple antibacterial mechanisms including acting upon bacterial cell membrane and cell wall. In conclusion, the species of sponge and coral collected from Malaysia have been shown to possess antimicrobial activity. RnD1c exhibited multiple antibacterial mechanisms including acting upon bacterial cell membrane and cell wall. These abilities highlighted it as a good lead compound for developing new antibiotics. This study commenced on Nov, 2008 and completed on Jan, 2012.en_US
dc.description.callnumbert QR 106 Q13A 2012en_US
dc.description.degreelevelDoctoralen_US
dc.description.identifierThesis : Antimicrobial activities of selected sponges and corals of costal waters of Malaysia /by Haitham Qarallehen_US
dc.description.identityt00011281611HaithamQarallehen_US
dc.description.kulliyahKulliyyah of Scienceen_US
dc.description.notesThesis (Ph.D)--International Islamic University Malaysia, 2012.en_US
dc.description.physicaldescriptionxx, 266 leaves : ill. ; 30cm.en_US
dc.description.programmeDoctor of Philosophy in Scienceen_US
dc.identifier.urihttps://studentrepo.iium.edu.my/handle/123456789/11203
dc.identifier.urlhttps://lib.iium.edu.my/mom/services/mom/document/getFile/jR8wR2UWDd1O82tVrFpQunBiTjJsG5fn20130920164918717
dc.language.isoenen_US
dc.publisherKuala Lumpur: International Islamic University Malaysia, 2012en_US
dc.rightsCopyright International Islamic University Malaysia
dc.subject.lcshSponges -- Microbiology -- Malaysiaen_US
dc.subject.lcshCorals -- Microbiology -- Malaysiaen_US
dc.subject.lcshCoastal ecology -- Malaysiaen_US
dc.subject.lcshAnti-infective agents -- Malaysiaen_US
dc.titleAntimicrobial activities of selected sponges and corals of costal waters of Malaysiaen_US
dc.typeDoctoral Thesisen_US
dspace.entity.typePublication

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