Publication:
Microencapsulation and characterization of Gentamicin-PLGA microsphere intended for orthopaedic infection

dc.contributor.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#en_US
dc.contributor.authorAhmad Fahmi bin Harun@Ismailen_US
dc.date.accessioned2024-10-09T07:22:38Z
dc.date.available2024-10-09T07:22:38Z
dc.date.issued2012
dc.description.abstractThe study was done for the purpose of developing biodegradable gentamicin-loaded microspheres fabricated using poly(D.L-lactic-co-glycolic acid) (PLGA). The microspheres were fabricated by manipulating several variables i.e. molecular weight of PLGA, types of surfactant/emulsifier, different concentrations of polyvinyl alcohol (PVA) as well as the oil phase and different HLB values of surfactants in order to achieve the best formulation for W /0/W emulsion during the fabrication process. Antibiotic treatment of orthopaedic infection is complicated by systemic toxicity and the need of effective therapeutic concentration necessary to ensure optimum killing of bacteria. To overcome the problem of systemic toxicity and to achieve a high initial release followed by sustained release of antibiotics, a new method of delivering gentamicin is attempted by encapsulating gentamicin into PLGA using multiple emulsion, solvent-evaporation method. Gentamicin was first extracted from the microspheres and quantified using Ninhydrin assay before the concentration was measured using UV spectrophotometer. Gentamicin loading after encapsulation was preserved when CTAB (83.51 ± 1.42%) and low molecular weight (LMW) PLGA (82.38 ± 9.08%) were used as indicated by drug loading of more than 80% in the discdiffusion assay. LMW PLGA enabled high burst release (-90%) of gentamicin within the first 10 hours corresponding to zone inhibition of 13.78 ± 0.86 mm, only 30% smaller than the positive control (10 mg/ml gentamicin). The effects of Tg and molecular weight rather than surfactant types influence the initial burst release. The in vitro release profile suggests that by having a mixture of various PLGA microspheres in one dosage implant system, the high burst release can be sustained within therapeutic concentration for a prolonged period (> 1 months). This biodegradable delivery system does not entail another surgery to remove the implant hence reducing the high treatment cost usually associated with the non-bidegradable proprietary gentamicin-polymethyl-methacrylate (PMMA) beads currently in use.en_US
dc.description.callnumbert RC 925.5 A2864M 2012en_US
dc.description.degreelevelMasteren_US
dc.description.identifierThesis : Microencapsulation and characterization of Gentamicin-PLGA microsphere intended for orthopaedic infection /by Ahmad Fahmi bin Harun@Ismailen_US
dc.description.identityt00011277037AhmadFahmiHarunen_US
dc.description.kulliyahKulliyyah of Pharmacyen_US
dc.description.notesThesis (MSPHT)--International Islamic University Malaysia, 2012.en_US
dc.description.physicaldescription[xix], 136 leaves :illustration ;30 cm.en_US
dc.description.programmeMaster in Pharmaceutical Sciences (Pharmaceutical Technology)en_US
dc.identifier.urihttps://studentrepo.iium.edu.my/handle/123456789/11175
dc.identifier.urlhttps://lib.iium.edu.my/mom/services/mom/document/getFile/qPWe6OhTejvsoQbmmTqW944CAH6OWc5R20180713103441281
dc.language.isoenen_US
dc.publisherKuantan, Pahang: International Islamic University Malaysia,2012en_US
dc.rightsCopyright International Islamic University Malaysia
dc.subject.lcshMusculoskeletal system -- Infections -- Treatmenten_US
dc.subject.lcshGentamicinen_US
dc.subject.lcshMicroencapsulationen_US
dc.titleMicroencapsulation and characterization of Gentamicin-PLGA microsphere intended for orthopaedic infectionen_US
dc.typeMaster Thesisen_US
dspace.entity.typePublication

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