In vitro maturation of oocytes using balb/c mice as a mammalian model for conservation of endangered species animals

Abstract

Conserving endangered species is a critical global challenge, with Assisted Reproductive Technologies (ART) playing a key role in preserving biodiversity. This study focuses on optimizing In Vitro Maturation (IVM) protocols using BALB/c mice (aged 8�16 weeks) oocytes as a model for endangered species conservation. The objectives include determining optimal doses of Follicle-Stimulating Hormone (FSH) and Luteinizing Hormone (LH) in IVM media, examining the effects of cumulus monolayer cells (CMC) and their secretions on oocyte maturation, and grading matured oocytes to evaluate the influence of different IVM environments. Findings indicate that specific FSH and LH concentrations significantly improve oocyte maturation rates. Co-culture with cumulus cells and supplementation with their secretions enhance oocyte quality and developmental potential. Oocyte evaluation, based on features such as the zona pellucida and polar body, provided key insights into effective IVM protocols. This study highlights the potential of refined IVM techniques as a non-invasive tool for increasing reproductive success in endangered species. The BALB/c mouse model proved valuable for developing species-specific IVM methods, which can be adapted for animals with similar reproductive traits. These advancements could improve genetic diversity and support conservation efforts for endangered wildlife, offering an ethical and efficient alternative to more invasive ART methods.