Publication:
Effect of gentamicin-nigella sativa fusion emulsions on osteoblast cell line for use in osteo healing-applications

cris.virtual.department#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.departmentcd0dd398-94b6-4652-a7a3-67d4a98f5e4b
cris.virtualsource.orcidcd0dd398-94b6-4652-a7a3-67d4a98f5e4b
dc.contributor.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#en_US
dc.contributor.authorFathin Athirah Yusof
dc.date.accessioned2024-10-08T07:25:19Z
dc.date.available2024-10-08T07:25:19Z
dc.date.issued2016
dc.description.abstractAn alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa oil (NSO) in a form of gentamicin-N. sativa fusion emulsion (GNFE). This work aims to formulate a stable emulsion and to study the effects of GNFE on UMR-106 osteoblast-like rat osteosarcoma cell line in vitro and its related mechanisms of bone healing and regeneration. Emulsion A, B, C and D had been formulated, with final concentration of gentamicin was made constant at 0.1%, whereas NSO concentration was varied at 32.5%, 35.0%, 40.2% and 46.4% in all formulations respectively. Stability studies of emulsion A, B, C and D were performed at different storage conditions (8°C, 25°C and 50°C), followed by in vitro study of MTT assay, Alizarin Red S (ARS) staining, von Kossa staining and quantification, alkaline phosphatase (ALP) quantification and quantitation of collagen type-1 and osteocalcin (qPCR). Results showed that all emulsions were stable at storage temperature of 8°C. In vitro results showed that emulsion D produced the highest cell viability (97.1%) at 72 hours of post-incubation. The highest mineral deposits (2.64 ± 0.05) and ALP activity (2.19 ± 0.3 nmol) was produced by emulsion D at day 21. Lastly, the highest expression of collagen type-1 (29.4 ± 1.01 folds) and osteocalcin (1.8 ± 0.51 folds) were expressed by the cells treated with emulsion C. Thus, stable GNFE may have the ability to promote bone formation.en_US
dc.description.callnumbert RM 666 G44 F252E 2016en_US
dc.description.degreelevelMaster
dc.description.identifierThesis : Effect of gentamicin-nigella sativa fusion emulsions on osteoblast cell line for use in osteo healing-applications/byFathin Athirah binti Yusofen_US
dc.description.identityt11100344023FathinAthirahen_US
dc.description.kulliyahKulliyyah of Allied Health Sciencesen_US
dc.description.notesThesis (MHSC)--International Islamic University Malaysia, 2016en_US
dc.description.physicaldescriptionxxvi, 165 leaves : ill. ; 30cm.en_US
dc.description.programmeMaster of Health Sciences (Biomedical Science)en_US
dc.identifier.urihttps://studentrepo.iium.edu.my/handle/123456789/9145
dc.identifier.urlhttps://lib.iium.edu.my/mom/services/mom/document/getFile/VGiiRBiySduqV9zweh343uJLLmhVgj2n20160329145550469
dc.language.isoenen_US
dc.publisherKuantan, Pahang : Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 2016
dc.rightsCopyright International Islamic University Malaysia
dc.subject.lcshGentamicinen_US
dc.subject.lcshBlack cumin -- Therapeutic useen_US
dc.titleEffect of gentamicin-nigella sativa fusion emulsions on osteoblast cell line for use in osteo healing-applicationsen_US
dc.typeMaster Thesisen_US
dspace.entity.typePublication
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#

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