Publication: Effect of gentamicin-nigella sativa fusion emulsions on osteoblast cell line for use in osteo healing-applications
cris.virtual.department | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
cris.virtual.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
cris.virtualsource.department | cd0dd398-94b6-4652-a7a3-67d4a98f5e4b | |
cris.virtualsource.orcid | cd0dd398-94b6-4652-a7a3-67d4a98f5e4b | |
dc.contributor.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | en_US |
dc.contributor.author | Fathin Athirah Yusof | |
dc.date.accessioned | 2024-10-08T07:25:19Z | |
dc.date.available | 2024-10-08T07:25:19Z | |
dc.date.issued | 2016 | |
dc.description.abstract | An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa oil (NSO) in a form of gentamicin-N. sativa fusion emulsion (GNFE). This work aims to formulate a stable emulsion and to study the effects of GNFE on UMR-106 osteoblast-like rat osteosarcoma cell line in vitro and its related mechanisms of bone healing and regeneration. Emulsion A, B, C and D had been formulated, with final concentration of gentamicin was made constant at 0.1%, whereas NSO concentration was varied at 32.5%, 35.0%, 40.2% and 46.4% in all formulations respectively. Stability studies of emulsion A, B, C and D were performed at different storage conditions (8°C, 25°C and 50°C), followed by in vitro study of MTT assay, Alizarin Red S (ARS) staining, von Kossa staining and quantification, alkaline phosphatase (ALP) quantification and quantitation of collagen type-1 and osteocalcin (qPCR). Results showed that all emulsions were stable at storage temperature of 8°C. In vitro results showed that emulsion D produced the highest cell viability (97.1%) at 72 hours of post-incubation. The highest mineral deposits (2.64 ± 0.05) and ALP activity (2.19 ± 0.3 nmol) was produced by emulsion D at day 21. Lastly, the highest expression of collagen type-1 (29.4 ± 1.01 folds) and osteocalcin (1.8 ± 0.51 folds) were expressed by the cells treated with emulsion C. Thus, stable GNFE may have the ability to promote bone formation. | en_US |
dc.description.callnumber | t RM 666 G44 F252E 2016 | en_US |
dc.description.degreelevel | Master | |
dc.description.identifier | Thesis : Effect of gentamicin-nigella sativa fusion emulsions on osteoblast cell line for use in osteo healing-applications/byFathin Athirah binti Yusof | en_US |
dc.description.identity | t11100344023FathinAthirah | en_US |
dc.description.kulliyah | Kulliyyah of Allied Health Sciences | en_US |
dc.description.notes | Thesis (MHSC)--International Islamic University Malaysia, 2016 | en_US |
dc.description.physicaldescription | xxvi, 165 leaves : ill. ; 30cm. | en_US |
dc.description.programme | Master of Health Sciences (Biomedical Science) | en_US |
dc.identifier.uri | https://studentrepo.iium.edu.my/handle/123456789/9145 | |
dc.identifier.url | https://lib.iium.edu.my/mom/services/mom/document/getFile/VGiiRBiySduqV9zweh343uJLLmhVgj2n20160329145550469 | |
dc.language.iso | en | en_US |
dc.publisher | Kuantan, Pahang : Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 2016 | |
dc.rights | Copyright International Islamic University Malaysia | |
dc.subject.lcsh | Gentamicin | en_US |
dc.subject.lcsh | Black cumin -- Therapeutic use | en_US |
dc.title | Effect of gentamicin-nigella sativa fusion emulsions on osteoblast cell line for use in osteo healing-applications | en_US |
dc.type | Master Thesis | en_US |
dspace.entity.type | Publication | |
oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# |