Publication:
Immobilization of Candida rugosa lipase on polymer support for monoacylglycerol production as halal emulsifier

cris.virtual.department#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.department141ffa33-6ee5-460b-a242-49b6a879067c
cris.virtualsource.orcid141ffa33-6ee5-460b-a242-49b6a879067c
dc.contributor.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#en_US
dc.contributor.authorHazimah Akbar Tajudin
dc.date.accessioned2024-10-08T05:42:56Z
dc.date.available2024-10-08T05:42:56Z
dc.date.issued2018
dc.description.abstractHalal emulsifier has a very high demand in the Halal industry market. The production of emulsifier using enzyme-based strategies provides an alternative solution for conventional emulsifier production. Due to the instability of biocatalyst, immobilization technology has been found to be a powerful tool to improve the performance of enzymes. The aim for this research is to optimize the immobilization conditions for Candida rugosa lipase attachment on poly (glycidyl methacrylate)-grafted-polyethylene/polypropylene microfibrous sheet (PGMA-g-PE/PP) through covalent bonding. A pretreatment was carried out on the polymer prior to immobilization and the amine group density on the chemically modified PGMA-g-PE/PP microfibrous sheet was found to be 3.33 mmol/g. The chemically modified microfibrous sheet was characterized by Fourier-transform infrared spectroscopy (FTIR-ATR) and field emission scanning electron microscope (FESEM). Response surface methodology (RSM) was applied to model and optimize the immobilization settings represented by three factors including immobilization time (2-6 h), pH (pH 7-9) and enzyme/support ratio (5.0-9.0 mg/cm2). A well-correlated significant model (p-value = 0.0003) was determined for the residual activity of the immobilized lipase (R2 = 0.9136). The enzymatic activity on p-nitrophenyl palmitate (pNPP) substrate achieved optimum under the conditions of 4.24 hrs, pH 8 and 8.51 mg/cm2 ratio of enzyme/support. The optimal reaction temperature and pH value in enzymatic reaction for both free and immobilized lipase were found to be 45 oC at pH 7 and 55 oC at pH 6, respectively. The pH endurance, storage and thermal stability of the immobilized lipase were remarkably enhanced. The immobilized lipase can be readily recovered and more than 50% of its activity was retained following 10 cycles. The kinetic parameters study showed that both Vmax and KM values were decreased from 0.16 mM/min and 4.69 mM to 0.15 mM/min and 2.86 mM, respectively after immobilization. Lastly, the immobilized lipase was evaluated for monoacylglycerol emulsifier production from Kenaf seed oil. Result showed that monoacylglycerol was detected by GC-TOF/MS. The results of this study suggested that the PGMA-g-PE/PP microfibrous sheet is a promising polymer support for enzyme immobilization with potential for Halal emulsifier production.en_US
dc.description.callnumbert BPH 124 H39 2018en_US
dc.description.degreelevelMasteren_US
dc.description.identifierThesis : Immobilization of Candida rugosa lipase on polymer support for monoacylglycerol production as halal emulsifier /by Hazimah binti Akbar Tajudinen_US
dc.description.identityt11100405063HazimahAkbarTajudinen_US
dc.description.kulliyahInternational Institute for Halal Research and Trainingen_US
dc.description.notesThesis (MSHIS)--International Islamic University Malaysia, 2018.en_US
dc.description.physicaldescriptionxv, 132 leaves :colour illustrations ;30cm.en_US
dc.description.programmeMaster of Science (Halal Industry Science).en_US
dc.identifier.urihttps://studentrepo.iium.edu.my/handle/123456789/8355
dc.identifier.urlhttps://lib.iium.edu.my/mom/services/mom/document/getFile/dyy72JJMKinGzkLHc6UjGHa4iSmBwTLs20200106120511482
dc.language.isoenen_US
dc.publisherKuala Lumpur :International Islamic University Malaysia,2018en_US
dc.rightsCopyright International Islamic University Malaysia
dc.subject.lcshHalal fooden_US
dc.subject.lcshImmobilized enzymesen_US
dc.subject.lcshEmulsionsen_US
dc.titleImmobilization of Candida rugosa lipase on polymer support for monoacylglycerol production as halal emulsifieren_US
dc.typeMaster Thesisen_US
dspace.entity.typePublication
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#

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