Extraction of fucoxanthin from selected Malaysian brown seaweeds and its effects on 3T3-L1 adipocyte cells

Ahmad, Hasna

Publication:
Extraction of fucoxanthin from selected Malaysian brown seaweeds and its effects on 3T3-L1 adipocyte cells

dc.contributor.affiliation	#PLACEHOLDER_PARENT_METADATA_VALUE#	en_US
dc.contributor.author	Ahmad, Hasna	en_US
dc.date.accessioned	2024-10-09T07:47:22Z
dc.date.available	2024-10-09T07:47:22Z
dc.date.issued	2017
dc.description.abstract	Obesity (BMI >30 kg/m2) is becoming a major public problem as it affects over 400 million of the population. Despite advances in the development of more effective weight loss drugs, safety and dependence remains an issue. This study aims to establish the antiobesity potential of Malaysian brown seaweeds Sargassum oligocystum, Padina australis and Dictytota dichotoma. Fucoxanthin has been described as the main bioactive compound in brown seaweed exerting antiobesity effect. Three extraction parameters were optimized in order to retain maximum fucoxanthin content in the extract: solvent, particle size and method of extraction. Optimal solvent and particle size for extraction across all seaweeds was acetone and particles sieved through 500?m mechanical sieve. S. oligocystum and P. australis were best extracted using Soxhlet procedures, while D. dichotoma was best extracted using maceration. The fucoxanthin content was analyzed using HPLC equipment. The highest amount of fucoxanthin present in the seaweeds was found in Sargassum oligocystum (754.8 mg/g dry weight), followed by D. dichotoma (142.9 mg/g) and finally P. australis extracts (91.58 mg/g). The extract showing best antiadipogenic effect on the preadipocytes was SAE (lipid accumulation equivalent to 0.170 ± 0.034 A), followed by DAE (0.196 ± 0.082 A) and finally PAE (0.203 ± 0.047 A). SAE had the best proadipolytic effect on mature lipid cells (31.86 ± 17.56 mg/ml glycerol release), followed by PAE (30.54 ± 15.41 mg/ml) and finally DAE (28.12 ± 11.36 mg/ml). Statistical analysis using one- way ANOVA indicated that the differences were not significant (p>0.05). Overall, antiobesity activity levels did not coincide with fucoxanthin levels present in the extract, indicating the presence of various compounds in the extract that exert diverse effects on 3T3-L1 adipocytes. The study describes fucoxanthin content of the Malaysian seaweeds and its optimal extraction parameters. Furthermore, it indicates the adipogenic and adipolytic potential of the extracts as antiobesity agents and the potential for seaweed extracts to be further developed as a safe and effective natural alternative for the management of obesity.	en_US
dc.description.callnumber	t RC 628 A286E 2017	en_US
dc.description.degreelevel	Master
dc.description.identifier	Thesis ; Extraction of fucoxanthin from selected Malaysian brown seaweeds and its effects on 3T3-L1 adipocyte cells /by Hasna Ahmad	en_US
dc.description.identity	t11100362151HasnaAhmad	en_US
dc.description.kulliyah	Kulliyyah of Science	en_US
dc.description.notes	Thesis (MSBTS)--International Islamic University Malaysia, 2017.	en_US
dc.description.physicaldescription	xiv, 115 leaves :illustrations ;30cm.	en_US
dc.description.programme	Master of Science in Biotechnology	en_US
dc.identifier.uri	https://studentrepo.iium.edu.my/handle/123456789/11365
dc.identifier.url	https://lib.iium.edu.my/mom/services/mom/document/getFile/CeEaygov2C52HoF5qJEclBf0HBYm8WLw20171030120312823
dc.language.iso	en	en_US
dc.publisher	Kuantan, Pahang :International Islamic University Malaysia,2017	en_US
dc.rights	Copyright International Islamic University Malaysia
dc.subject.lcsh	Obesity -- Alternative treatment	en_US
dc.subject.lcsh	Marine algae -- Malaysia	en_US
dc.subject.lcsh	Bioactive compounds	en_US
dc.title	Extraction of fucoxanthin from selected Malaysian brown seaweeds and its effects on 3T3-L1 adipocyte cells	en_US
dc.type	Master Thesis	en_US
dspace.entity.type	Publication