Antimicrobial investigation of crude extracts and purified compounds of Garcinia Griffithii T.Anders and Artocarpus Altilis (Parkinson) fosberg,Malaysian tropical plants

Abstract

The present study focuses on the bioassay guided isolation of antimicrobial compounds from Garcinia griffithii and Artocarpus altilis. The hexane, ethyl acetate, dichloromethane and methanol extracts were obtained from the dried (leaves of G. griffithii and twigs of A. altilis) by using Soxhlet extraction. The plants crude extracts were evaluated for in vitro antimicrobial activity against six pathogenic microorganisms (two Gram-positive strains: Staphylococcus aureus (ATCC-25923) and Bacillus cereus (ATCC-11778), two Gram-negative strains Escherichia coli (ATCC-35218) and Pseudomonas aeruginosa (ATCC-27853), and two fungal strains: Candida albicans (ATCC-10231) and Cryptococcus neoformans (ATCC-90112)) using the disc diffusion method at concentration of 2 mg/disc. The antimicrobial activity of G. griffithii methanol extract showed the highest zone of inhibition of 18±2.4mm, while hexane and dichloromethane extracts of A. altilis was 14.6±0.2mm. Whereas minimum inhibition concetration (MIC) and minimum bactericidal/minimum fungicidal concetration (MBC/MFC) for isolated compounds and the extracts were ranged from 62.5-500 μg/ml and 250-2000 μg/ml, respectively. Bioassay guided isolation of the methanol extract of G. griffithii afforded betulinic acid and from hexane and dichloromethane (DCM) extracts of A. altilis afforded cycloartenyl acetate and stigmasterol respectively. Their structures were identified by 1H-NMR, 13C-NMR, IR and the spectra obtained were compared with previous data. This study forms a strong basis for declaring G. griffithii and A. altilis as antimicrobial potential sources to treat pathogenic microorganisms.